Growth kinetics and immune cell profiling of BT-474 human breast carcinoma and Raji-Luc human Burkitt's lymphoma in four strains of triple immunodeficient mice

AACR 2023 -- Triple immunodeficient mouse models are often necessary to evaluate xenograft models and adoptive cell therapies using human immune cells. Triple immunodeficient mice lack functional T cells, B cells and natural killer (NK) cells. Female NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice, NOD.CB17-Prkdcscid IL2rgtm1/BcgenHsd (B-NDG) mice, NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl (NCG) mice and NOD.Cg-Prkdcscid Il2rgtm1Sug/JicTac (CIEA NOG) mice were implanted with subcutaneous BT-474 or systemic Raji-Luc to evaluate growth kinetics and to collect samples to assess immune cell profiles. Disease induction and growth parameters were determined through previous experience and optimization studies with subcutaneous BT-474 and systemic Raji-Luc (data not shown). Blood, spleen and tumor samples were collected from mice implanted with BT-474 and blood, spleen and bone marrow samples were collected from mice implanted with Raji-Luc. Immune cell profiles were analyzed via flow cytometry to quantify lymphocyte and myeloid immune cell subsets and to observed differences between the four mouse strains.

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Preclinical & Nonclinical