Human and Mouse Reconstructed Plate-Based 3-Dimensional Culture Assays That Mimic the Tumor Microenvironment

AACR 2021 -- Why are 3D cell culture models more important and relevant than standard 2D models? 2D models depict the cells in an unnatural state, since they are cultured and scaled-up using rigid plastic surfaces. Cells assayed in a 2D format are limited in that they take on a different physiology, polarized in the expression of cell surface proteins like integrins and receptors and their morphology takes on a flattened cell shape. Carcinoma cells quickly overgrow the wells they are plated in and either become senescent or apoptotic. Despite these drawbacks, 2D cultures are the current model of choice for drug screening due to their low cost and simplicity. The 3D culture system developed by zPREDICTA recapitulates the tumor cell microenvironment. This allows the cancer cells to behave like cancer cells in the stroma and scaffolding of the organ-specific matrix. The cells migrate, proliferate, form tumor-like clusters and show a high correlation with clinical responses.

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