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Labcorp is a leading global life sciences company that includes contract research and developmental services to the pharmaceutical, medical technology, crop protection and chemical industries.
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Characterization of Orthotopically Implanted Syngeneic Cell Lines as More Clinically Relevant In Vivo Models
AACR 2021 -- Preclinical oncology studies aim to model human disease in animal settings. To this effect, the site of tumor implant is thought to impact critical parameters that determine response to treatment. The more frequently used subcutaneous (SC) models have advantages: speed, cost efficiency and ability to monitor growth by caliper measurements but they lack heterogeneous tumor morphology, vessel density and immune cell infiltrates comparable to human disease. Orthotopic (OT) tumor models allows for tumor modeling in the organ of origin by mimicking many human disease parameters such as vascularization, tumor architecture, metastatic potential, immune profile and tumor microenvironment. By virtue of this, OT models may better represent clinical disease, but are more challenging to monitor in vivo. Thus, we stably transfected several mouse syngeneic cell lines with luciferase (Luc). The Luc-enabled cells facilitate non-invasive monitoring of tumor progression, potential metastasis and response to treatment via bioluminescence imaging (BLI). Here, we focus on pancreas (Pan02-Luc) and lung (LL/2-Luc) models and show their response to immune modulators and standard-of-care agents in the OT setting. Additionally, we also show data with the parental lines in the SC setting.
