Cell sorting using flow cytometry is a specialized type of flow cytometry. It provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell. Cell sorting can separate live cells based on any scatter, fluorescent signals, or combination of both. We have experience separating live vs. dead cells, cells expressing a specific fluorescent protein, or cells expressing a specific surface marker or set of surface markers. After separation, cells can be cultured and/or used in any downstream assay. We have also successfully created multiple single cell clones expressing different levels of specific proteins from multiple cell lines. We collaborate with a partner laboratory that has multiple cell sorting flow cytometers and years of experience sorting cells in multiple ways.