Apoptosis is a normal physiologic process, the program of which is characterized by certain morphologic features, including loss of plasma membrane asymmetry and attachment, condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA. FITC Annexin V staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either apoptotic or necrotic processes. Therefore, staining with FITC Annexin V is typically used in conjunction with a vital dye such as propidium iodide (PI) to identify early apoptotic cells (PI negative, FITC Annexin V positive). Viable cells with intact membranes exclude PI, whereas the membranes of dead and damaged cells are permeable to PI. Let us determine if treatment with your compound includes apoptosis in your samples and receive data regarding the percentage of cells in each sample that are alive, apoptotic, and dead. Our flow cytometry team can perform apoptosis assays on multiple types of samples such as cultured cells, blood, or tissues, and, if desired, analyze specific subsets of cells within each sample.