Acute myelogenous leukemia (AML) is a rapidly progressing malignancy of myeloid white blood cells. An estimated 21,380 new cases of AML and 10,590 deaths will occur in the United States in 2017. AML results from excessive proliferation of dedifferentiated or undifferentiated myeloid leukocytes initially in the bone marrow. As disease progresses, AML blast cells will be found in the blood and other secondary lymphoid organs. Symptoms including anemia and leukopenia result from proliferating AML cells suppressing normal hematopoiesis in the bone marrow. AML is characterized by a complex number of subtypes that are defined by genomic alterations and gene expression profiling. Treatment typically starts with intensive chemotherapy followed by allogeneic bone marrow transplantation (BMT) in patients that can tolerate these therapies. Less debilitating treatment options, including immunotherapy, are needed for older AML patients who frequently cannot tolerate intensive chemotherapy or BMT.
C1498 is a murine AML cell line that arose spontaneously in a C57BL/6 mouse and grows aggressively in syngeneic mice. Our C1498 line has been luciferase- and mCherry-enabled to permit monitoring of whole body tumor growth by bioluminescence imaging (BLI) and quantification of tumor burden in isolated tissues by flow cytometry, respectively. Intravenous implantation of C1498-luc-mCherry into syngeneic C57BL/6 mice results in progressive growth of the cells as monitored by BLI (Figure 1A) with signal evident in the long bones (bone marrow) and other disseminated sites during disease progression (Figure 1B).
Growth Kinetics and Representative BLI Images