Figure 2: Tumor cells were implanted into the mammary fat pad of female Balb/C mice. At a mean tumor volume of ~100-150mm3 mice were untreated or treated with a single dose of 5, 10 or 20Gy as indicated on day 7. A, group mean tumor volumes over time. B, individual tumor volumes for untreated control mice over time. C, individual tumor volumes for 5Gy treated mice over time. D, individual tumor volumes for 10Gy treated mice over time. E, individual tumor volumes for 20Gy treated mice over time. In B-E the black dashed line indicates mean tumor volume of untreated group over time. In C-E, the colored dashed line is the mean tumor volume of the indicated group.
Body weight loss was only observed in mice that exhibited advanced disease associated with lung and/or thoracic area metastasis that the model is known to produce. In Figure 3, percent change in body weights over time showed toleration of all three doses of radiation during the course of the study. In order to best utilize this model for radiation combination studies, we recommend using the moderately active 10Gy dose which allows for an ample therapeutic window to combine with novel therapies.