Fig. 2B: Bone CT imaging.
Orthotopic and Metastatic Modeling
The PC-3M-Luc-C6 model, by virtue of its luciferase tag, allows for longitudinal monitoring of disease by bioluminescence imaging (BLI). This model is ideal for studies investigating response of orthotopic or metastatic disease upon treatment of a drug candidate.
To initiate PC-3M-Luc-C6 as an orthotopic model, cells are surgically implanted into the prostate gland, and a signal in that location demonstrates established disease within two weeks of implant (Figure 3). Animals will succumb to disease between Days 50 and 65 post implant, which enables at least six weeks of dosing. In late stage disease following orthotopic implant, abdominal distension is common (~50-90%) due to fluid accumulation in the abdominal space. Metastasis within the peritoneal space, including liver, spleen, intestine, and abdominal wall can also occur in a small number of animals (30%), but bone metastasis has not been recorded as a result of orthotopic prostate implantation.
Tumor Progression Following Orthotopic PC-3M-Luc-C6 Implant